Variola Virus-Specific Diagnostic Assays: Characterization, Sensitivity, and Specificity

Correction for Kondas et al., Variola Virus-Specific Diagnostic Assays: Characterization, Sensitivity, and Specificity.

A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.

Characterization of sensitivity and specificity in leaky droplet-based assays.

This paper uses numerical methods to characterize the crosstalk of small fluorescent molecules and molecular probes among aqueous droplets immersed in a continuous phase of hydrocarbons or fluorocarbons in microfluidic systems. Droplet-based biochemical assays rely on the reagents to remain isolated in individual droplets. It has been observed, however, that small and hydrophobic fluorescent mo...

Specificity and performance of diagnostic PCR assays.

a systematic review on diagnostic procedures for specific language impairment: the sensitivity and specificity issues

background: identification of children with specific language impairment (sli) has been viewed as both necessity and challenge. investigators and clinicians use different tests and measures for this purpose. some of these tests/measures have good psychometric properties, but it is not sufficient for diagnostic purposes. a diagnostic procedure can be used for identification a specific population...

Diagnostic Utility of Chest X-rays in Neonatal Respiratory Distress: Determining the Sensitivity and Specificity

Background: Chest radiography is one of the most usual diagnostic tools for respiratory distress. Objective: The purpose of this study is to assess the specificity, sensitivity and clinical value of chest radiography of neonates with respiratory distress.Patients and Methods: A descriptive- analytical study was conducted on 102 neonates that were in neonatal intensive care unit of Imam Reza and...